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大鼠海马发育过程中netrin-1、nogo-A和tsp-1 mRNA的表达

Expression of netrin-1,nogo-A and tsp-1 mRNA in the development of rat hippocampus
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摘要 目的:探讨神经元突起定向生长和突触形成过程的规律。方法:采用RT-PCR方法检测大鼠发育不同阶段胶质细胞源性netrin-1、nogo-A和tsp-1 mRNA的表达。结果:RT-PCR实际扩增长度与设计长度相吻合,未出现与DNA相同的杂带。内参照β-actin的PCR产物电泳条带在胚胎、新生和成年时灰度较均一。netrin-1 mRNA以胎鼠和新生大鼠表达较多,成年大鼠弱表达(P<0.01)。nogo-A在各个阶段均呈高表达,以胎鼠和新生大鼠较多,其中新生大鼠表达最多(P<0.01),成鼠相对较低(P<0.01)。tsp-1以胎鼠和新生大鼠表达较多,成年大鼠表达微弱(P<0.01)。结论:大鼠海马胚胎和新生后是突起定向生长的主要阶段,抑制突起的生长则发生在发育的各个阶段,以新生后为主,突触结构和功能的发育发生在胚胎和新生后阶段。 Aim: To investigate the rule of neurite orientating growth and synapse formation.Methods: The mRNA of netrin-1,nogo-A and thrombospondins-1(tsp-1) was detected using RT-PCR.Results: Limpid electrophoresis strips located in the contrived position,and no hetero-strips appeared.The expression of β-actin was similar in all rats.The most expression of netrin-1 was in embryonic and neogenetic rats,and the least was in adult rats(P<0.01).The expression of nogo-A was more in all period and mainly in the embryoni...
作者 郭国庆 辛莉 沈伟哉 邱小忠 钟世镇
机构地区 南方医科大学临床解剖学研究所广东省组织构建与检测重点实验室 暨南大学医学院解剖学教研室
出处 《暨南大学学报（自然科学与医学版）》 CAS CSCD 北大核心 2006年第4期546-550,共5页 Journal of Jinan University(Natural Science & Medicine Edition)
基金 广州市科技计划项目(2004J1-C0241) 广东省自然科学基金团队项目(039213)
关键词 NETRIN NOGO-A TSP-1 海马 大鼠 netrin-1 nogo-A thrombospondins-1 hippocampus develop-ment rat
分类号 Q42 [生物学—神经生物学]
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参考文献8

  • 1[1]KAPRIELIAN Z,IMONDI R,RUNKO E.Axon guidance at the midline of the developing CNS[J].Anat Rec,2000,261 (5):176-197.
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二级参考文献6

  • 1Chen MS, Huber AB, van der Haar ME, et al. Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature, 2000,403(6768):434-439.
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  • 3Jin WL, Liu YY, Liu H. Intraneuronal localization of Nogo-A in the rat. J Comp Neurol, 2003,458(1):1-10.
  • 4Gianola S, Savio T, Schwab ME, et al. Cell-autonomous mechanisms and myelin-associated factors contribute to the development of Purkinje axon intracortical plexus in the rat cerebellum. J Neurosci, 2003,23(11):4613-4624.
  • 5Weiss J, Takizawa B, McGee A, et al. Neonatal hypoxia suppesses oligodendrocyte Nogo-A and increases axonal sprouting in a rodent model for human prematurity. Exp Neurol, 2004,189(1):141-149.
  • 6Tozaki H, Kawasaki T, Takagi Y, et al. Expression of Nogo protein by growing axons in the developing nervous system. Brain Res Mol Brain Res, 2002,104:111-119.

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暨南大学学报（自然科学与医学版）
2006年 第4期

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