两种球形棕囊藻细胞总RNA提取方法的比较

Comparison of two methods of total RNA isolation from Phaeocystis globosa

不同植物细胞RNA提取的适宜方法各不相同.本实验分析、比较了QIAGEN试剂盒法和西曲溴铵(CTAB)法用于赤潮藻细胞总RNA提取的情况.结果显示,两种方法均可获得高质量的总RNA.比较而言,CTAB法比QIAGEN试剂盒更可取,适合藻细胞RNA的大量提取.

The methods of total RNA isolation are varied for the different plant cells.In this paper,two methods of total RNA isolation,QIAGEN total RNA isolation kit and CTAB isolation buffer solution,were compared when used for red algal cells.It was found that both the two methods could isolate good total RNA.However,CTAB method is better suitable for the substantive RNA isolation from red tide algae.