血小板上清液对家兔生长板软骨细胞增殖的影响

The study of human platelet supernatant on the proliferation of rabbit growth-plate chondrocytes

目的:观察人血小板上清液(hum an p latelet supernatant,hPS)对体外培养幼兔生长板软骨细胞增殖与分化的影响。方法:原代分离培养兔生长板软骨细胞并稳定传代,同时添加不同体积分数的hPS,采用W ST-8染色法检测细胞增殖倍数;A lc ian B lue法测基质蛋白多糖含量;羟脯氨酸法测定软骨细胞胶原产量,酶动力学方法测定碱性磷酸酶(ALP)活性。结果:体积分数5%hPS组显著促进软骨细胞的增殖以及基质蛋白多糖的合成,但形态上失去其软骨表型。各体积分数组hPS对总胶原的合成与对照组相比差异没有显著性意义,hPS抑制了ALP的活性。结论:hPS可以有效地促进生长板软骨细胞的增殖,抑制其肥大化。

Aim: This study aimed at the biological effect of human platelet supernatant(hPS) on the rabbit growth-plate chondrocytes.Methods: Growth-plate chondrocytes were isolated from rabbit costal cartilage and expanded under low serum condition and hPS in monolayers.Cells growth was detected by improved MTT;The production of proteoglycans and the total collagen was determined by Alcian Blue method and the hydroxyproline content assay respectively.ALP activity was measured by modified enzyme dynamical method.Resul...