枸骨叶不同溶媒萃取物对小鼠体外T淋巴细胞活化增殖的影响

Effect of chemical extraction agents from Ilex latifolia Thunb on mouse T lymphocytes activation and proliferation in vitro

目的:探讨枸骨叶5种溶媒萃取物对T淋巴细胞增殖、活化的影响。方法:利用MTT法检测枸骨叶对体外ConA刺激T淋巴细胞增殖反应及使用流式细胞仪测定CD3阳性淋巴细胞的CD69分子表达的影响。结果:枸骨叶的醇提物I、乙酸乙酯IⅠ、正丁醇萃取物IⅡ具有明显抑制Co-nA刺激T淋巴细胞增殖反应的作用。枸骨叶醇提物I与乙酸乙酯萃取物IⅠ能明显抑制ConA刺激T淋巴细胞CD69分子的表达。枸骨叶2种水提物无免疫抑制作用。结论:枸骨叶脂溶性萃取物具有较强抑制T淋巴细胞活化、增殖的化学成分。

Aim: To study the effects of five kinds of chemical extraction agents from Ilex latifolia Thunb on mouse T lymphocytes activation and proliferation in vitro.(Methods:)The effect of five chemical extraction agents(sample I,I_Ⅰ,I_Ⅱ,I_Ⅲ,II) from Ilex latifolia Thunb on proliferation of murine lymphocytes and the expression level of CD69 on T cells stimulated by ConA were analysed by MTT assay or flow cytometry respectively.Results:Sample I、sample I_Ⅰand sample I_Ⅱ can inhibit the proliferation of murine lympho...