机械损伤对富士苹果抗氧化酶活性的影响

Effects of mechanical damage on the activites of antioxidant enzymes in fuji apples

研究富士苹果遭受机械损伤后在两种贮藏温度下伤害部位超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)等酶活性的变化,并对抗坏血酸(AsA)、还原性谷胱甘肽(GSH)等内源抗氧化剂的含量进行分析测定。试验结果表明:与对照果实相比,受伤果实的SOD活性、APX活性在贮藏初期逐渐下降,GR活性迅速下降后又上升,CAT活性、POD活性逐渐上升;同时,作为抗氧化剂GSH含量与AsA含量与GR活性保持了相同的变化趋势;随着贮藏时间的延长,各种抗氧化酶活性和抗氧化剂含量又呈现上升和下降的反复过程,而且温度越低,各种生理指标变化越缓慢,且显示较高的酶活性和较高的抗氧化剂含量。表明机械损伤显著地诱导了抗氧化酶活性和抗氧化剂含量的变化,以达到提高受伤组织自身对伤害的修复能力,而且在低温下,受伤部位具有较高的对伤胁迫的修复能力。

The changes in the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR) and the contents of ascorbic acid (AsA), reduced glutathione (GSH) of the wounded site of Fuji apples by mechanical damage during storage at 5 ℃ and 18 ℃ were investigated. The results showed that the activities of SOD and APX of the injured fruit compared with that of the control decreased gradually during prophase storage, the activities of GR decreased rapi...