灯盏花素注射液中非法添加物的研究初探

Study on the illegal additive in TCM breviscapine injection

目的:分析中成药灯盏花素注射液中的非法添加物。方法:通过理化鉴别、薄层色谱法、紫外分光光度法、高效液相色谱法等方法初步对添加物进行定性,采用HPLC方法对添加物的含量进行测定。结果:非法添加物呈维生素C鉴别的阳性反应;且维生素C进样量与峰面积在0.1004～1.0040μg范围内,呈良好的线性关系,回归方程Y=3006.7x-9.5449,r=0.9998(n=6);平均回收率为102.05%(n=6)。结论:灯盏花素注射液中非法添加维生素C,并测得其含量为8.0858mg/ml。

Objective: To analyse the illegal additive in TCM breviscapine injection. Method: Methods of chemical reactions ,TLC,UV-Spectrophotometry and HPLC, were adopted for the qualitative analysis. Results: Chemical reactions which were applied to test Vc were positive .There was a good relationship when the content of VitC was 0.1004～1.004 μ(r=0.9998).The average recovery was 102.05%. Conclusion: The experimental results indicated that: VitC was added to the injection illegally ,and the content of VC was 8.0858mg...