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丙戊酸钠联合三氧化二砷对HL-60细胞株的体外研究 被引量：5

Study of the combination of sodium valproate and arsenous trioxide on HL-60 cells
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摘要 目的:探讨丙戊酸钠(sodium valproate,SV)联合三氧化二砷(arsenous trioxide,As2O3)诱导HL-60细胞凋亡与bcl-2﹑bax mRNA之间可能的关系。方法:在对数生长期的HL-60细胞中分别加入不同浓度的SV和1.0μmol/LAs2O3,采用MTT比色法测定细胞生长抑制率,用Annexin V-FITC/PI双重标记经流式细胞仪检测细胞早期凋亡率,RT-PCR检测bcl-2,bax mRNA在细胞中的表达。结果:SV抑制HL-60细胞生长并促进其凋亡,SV联合1.0μmol/L As2O3对HL-60细胞的生长抑制及促凋亡作用更明显。SV单独或联合1.0μmol/L As2O3作用于HL-60细胞,bcl-2mRNA表达下降,bax mRNA表达增高。结论:SV与As2O3有协同作用,抑制HL-60细胞生长并诱导其凋亡,bcl-2﹑bax mRNA的表达变化可能参与了SV对HL-60细胞的促凋亡作用。 Objective: To study the growth inhibition and apoptosis of HL-60 cells affected by the combination of sodium valproate (SV) and arsenous trioxide (As2O3), and to explore the relationship between the apoptosis of HL-60 cells induced by the combination of SV and As2O3 and the expression of bcl-2 and bax mRNA. Methods: Various concentrations of SV and 1.0 μmol/L As2O3 were added to exponential growth HL-60 cells . MTT assays were used to measure the growth inhibitory effect of combination of SV and As2O3. Flow...
作者 张洁 滕镕
机构地区 南通大学附属医院血液内科
出处 《南通大学学报（医学版）》 2008年第1期20-22,共3页 Journal of Nantong University(Medical sciences)
关键词 丙戊酸钠 三氧化二砷 HL-60细胞 凋亡 Bcl-2 BAX Sodium valproate Arsenous trioxide HL-60 cells Apoptosis Bcl-2 Bax
分类号 R733.7 [医药卫生—肿瘤]
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参考文献7

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同被引文献39

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南通大学学报（医学版）
2008年 第1期

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