摘要
目的:探讨移植的NSCs在大鼠脑损伤区壳聚糖载体中的存活、分化情况及其对TBI大鼠认知功能的影响。方法:低温冷冻干燥法制备壳聚糖多孔支架。将从鼠胚前脑中分离的NSCs扩增、标记BrdU。Feeney法制备SD大鼠TBI模型,随机分为3组:损伤对照组清创后不做移植;NSCs+支架移植组行壳聚糖作载体的NSCs移植;NSCs+支架+NGF移植组行壳聚糖作载体的NSCs移植,并在其中加入NGF。术后1、2、3个月行避暗回避和跳台试验。脑切片行Nissl染色、BrdU与NF-200免疫荧光双标染色。结果:两移植组的认知功能在术后1、2、3个月较损伤对照组明显改善,含NGF的移植组改善更加显著。两移植组术后1、2、3个月在移植区均可见BrdU与NF-200免疫荧光双标细胞,含NGF移植组中的双标细胞数量多、胞体大、突起多且长。结论:大鼠TBI后移植的外源性NSCs可以在脑损伤区壳聚糖载体中长期存活并向神经元分化,可以改善大鼠的认知功能;NGF对其具有促进作用。
Objective: To explore the survival and differentiation of transplanted NSCs in the chitosan porous scaffold within the cerebral cortex lesion of rats and the influence on the cognitive function of rats with TBI. Methods: The porous chitosan scaffold was made by Freezing-drying technique. The NSCs coming from rat fetal forebrain were labeled with BrdU. Sprague-Dawley(SD) rats TBI models were made by Feeney s method. The TBI rats were randomly divided into injury-control group, NSCs+scaffold group and NSCs+s...
出处
《南通大学学报（医学版）》
2008年第1期12-16,共5页
Journal of Nantong University(Medical sciences)
基金
南通市社会发展科技计划项目(S40014)
关键词
创伤性脑损伤
壳聚糖
神经干细胞
移植
神经元
神经生长因子
Traumatic brain injury
Chitosan
Neural stem cells
Transplantation
Neuron
Nerve growth factor
