应用噬菌体检测对虾发光病致病菌哈维氏弧菌的可行性研究

Feasibility Study of Using Bacteriophage to Detect Penaeid Shrimp Luminous Disease Pathogen,Vibrio Harveyi

探讨了应用哈维氏弧菌噬菌体检测对虾发光病致病菌哈维氏弧菌(Vibrio harveyi)的方法和可行性.实验表明,从对虾育苗场分离的哈维氏弧菌噬菌体对对虾发光病致病菌哈维氏弧菌的噬菌作用具有相当高的特异性,它不能裂解同样来源的溶藻弧菌、副溶血性弧菌、光合细菌,却能特异地裂解本实验所用的不同来源的哈维氏弧菌,噬菌斑出现率可达80%~100%,并且噬菌斑的出现率与虾苗发生发光病的发病程度有一定的相关性,可应用于哈维氏弧菌的检测和对虾发光病致病菌的诊断.

This paper presents a feasibility study of using bacteriophage to detect penaeid shrimp luminous disease pathogen,Vibrio Harveyi.The results indicated that the Vibrio harveyi bacteriophage separated from shrimp hatchery showed a special phagocytosis on Vibrio harveyi.They could not infect and lyses certain bacteria such as Vibrio alginolyticus,Vibrio parahaemo-lyticus and photosynthetic bacteria,but could specially lyse the experimental Vibrio harveyi which were separated from the same hatchery.The appearan...