摘要
目的建立胎鼠背根神经节(dorsal root ganglion,DRG)神经元原代培养的方法。方法无菌条件下取E16天的胎鼠DRG进行原代培养,观察神经元生长状态并用神经元特异性烯醇化酶(neuronal specific enolase,NSE)免疫细胞化学染色鉴定细胞。结果培养的DRG神经元可存活1个月左右,并长出突起,形成密集的网络。NSE鉴定细胞阳性表达率高,神经元达到90%左右纯度。结论本文建立了DRG神经元细胞简洁、经济、高效的培养方法并成功鉴定了神经元。为对神经元的深入研究提供了实验模型。
Objective To establish a culture system of dorsal root ganglion neurons derived from embryonic rat.Method Dorsal root ganglion neurons harvested from E16 rats were produced into single cell suspension,then cultured dorsal root ganglion neurons.The purificational rates were evaluated according to neuronal specific enolase(NSE) immunocytochemistry stain.Result Cultured dorsal root ganglion neurons could survive for about 1 month and extend the process which formed dense net work.The neuron purity was high.The...
出处
《石河子大学学报（自然科学版）》
CAS
2007年第5期596-598,共3页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金资助项目(30160026)
