新疆艾比湖中度嗜盐菌A6的16SrDNA分析

Analysis of 16SrDNA of a Moderately Halophilic Bacteria A6 from Xinjiang Aibi Lake

从新疆艾比湖分离得到中度嗜盐菌A6,该菌能够在0%~20%NaCl的条件下生长,为有芽孢的杆状菌。采用真细菌16SrDNA通用引物PCR扩增A6菌株的16SrDNA,得到的片段长度为1512bp。使用NCBI^Blast软件在Gen-bank数据库中对其进行同源性检索,A6的16SrDNA序列与多种地衣芽孢杆菌16SrDNA序列同源性高达99%。使用MEGA3.1软件构建系统发育树,A6菌株与地衣芽孢杆菌的亲缘关系最近。由此判断A6菌株属于芽胞杆菌属。结合生理生化分析及对A6菌株的甜菜碱醛脱氢酶基因和Ⅲ型乙醇脱氢酶基因的序列同源性分析,初步确定A6菌株有可能是地衣芽胞杆菌的1个新种。

The moderately halophilic bacteria A6 was isolated from Xinjiang Aibi Lake.Strain A6 can grow on the media containing 0%～20% NaCl,and was bacilli with sporeforming.The common primers for the 16SrDNA of eubacteria were used for polymerase chain reaction.The amplified fragment length was 1512bp.Homology analysis was made by searching in Genbank with NCBI-Blast.The result indicated that the 16SrDNA homologies were 99% between strain A6 and many kinds of Bacillus Licheniformises.The phylogenetic tree was constr...