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烟草硫氧还蛋白基因NtTRX-hl的克隆和功能分析 被引量：1

Cloning and Functional Analyses of a Tobacco Thioredoxin Gene
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摘要 从烟草中克隆了硫氧还蛋白基因NtTRX-hl并推导出其氨基酸序列,系统进化关系分析表明,NtTRX-hl与小麦、大麦、拟南芥等多种植物的TRX蛋白有很高的同源性.构建高效表达载体,诱导表达重组菌得到分子大小为22.26 kD的融合蛋白,与推测的分子量一致.用胰岛素和二硫苏糖醇作底物进行酶活性测定表明,Nt-TRX-hl具有活性.采用生物信息学的方法和工具分析了NtTRX-hl蛋白的生化参数和亚细胞定位,预测了该蛋白的高级结构. The gene encoding a thioredoxin h-like(NtTRX-hl) protein from Nicotiana tobacum is cloned and function of the NtTRX-hl protein studied.Phylogenetic relationships between NtTRX-hl and homologues from other plant species are evaluated,indicating a particular relevance of the gene in plants.NtTRXhl protein is produced by recombinant Escherichia coli cells.Using dithiothreitol and insulin as substrate,NtTRX-hl shows evident enzymatic activity.Subcellular localization and three-dimensional structure of NtTRX-hl ...
作者 宋韬 任海英 吴廷全 孙力军 顾甘雨 董汉松
机构地区 南京农业大学植物保护学院
出处 《鲁东大学学报（自然科学版）》 2007年第3期256-260,共5页 Journal of Ludong University:Natural Science Edition
基金 教育部科学技术研究重点项目(03170)
关键词 烟草 硫氧还蛋白 基因克隆 功能分析 tobacco thioredoxin gene cloning functional analysis
分类号 Q943.2 [生物学—植物学]
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参考文献19

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同被引文献10

  • 1曹志亮,文喻呤,陈元鼎.A组人轮状病毒VP6基因克隆及在大肠杆菌中的高效表达[J].中国生物工程杂志,2006,26(3):37-41. 被引量：12
  • 2GELHAYE E,ROUHIER N,JACQUOT J P.The plant thioredoxin system[J].Cellular and Molecular Life Sciences,2005,62(1):24-35.
  • 3GELHAYE E,ROUHIER N,JACQUOT J P.The thioredoxin h system of higher plants[J].Plant Physiology and Biochemistry,2004,42(4):265-271.
  • 4PETERSON F C,LYTLE B L,SAMPATH S,et al.Solution structure of thioredoxin hi from Arabidopsis thaliana[J].Protein Science,2005,14(8):2195-2200.
  • 5SCHURMANN P.Redox signaling in the chloroplast:the ferredoxin/ thioredoxin system[J].Antioxid Redox Signal,2003,5(1):69-78.
  • 6HOLMGREN A.Thioredoxin catalyzes the reduction of insulin disulfides by dithiothreitol and dihydrolipoamide[J].The Journal of Biological Chemistry,1979,254 (19):9627-9632.
  • 7孙力军.硫氧还蛋白与生物激发子对植物抗病性的调控与诱导作用[D].南京:南京农业大学,2008.
  • 8COUDEVYLLE N,THUREAU A,HEMMERLIN C.Solution structure of a natural CPPC active site variant,the reduced form of thioredoxin h1 from poplar[J].Biochemistry,2005,44(6):2001-2008.
  • 9KOH C S,NAVROT N.An atypical catalytic mechanism involving three cysteines of thioredoxin[J].The Journal of Biological Chemistry,2008,283(34):23062-23072.
  • 10REICHHELD J P,CHRISTOPHE L,YVES M.The multigenic family of thioredoxin h in Arabidopsis thaliana:specific expression and stress response[J].Plant Physiology and Biochemistry,2002(40):685-690.

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鲁东大学学报（自然科学版）
2007年 第3期

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