温度对珍稀极危水生植物中华水韭大孢子萌发率的影响

Effects of Temperature on the Megaspores′ Germination of the Rare and Critically Endangered Aquatic Plant Isoetes sinensis

为进一步了解中国一类保护的水生极危野生植物中华水韭以及在大量人工繁殖和迁地保护等方面提供更为详实的证据,运用无菌培养、快速繁殖的方法对中华水韭的大孢子萌发率作了统计,探讨了不同温度对其大孢子萌发的影响.结果显示:中华水韭大孢子的萌发率在培养后第8~10周达到高峰,最高萌发率为47%,最佳萌发条件为低温4℃预处理5d后20℃恒温光照培养.在已萌发的雌配子体中,幼苗的形成率仅为12%,模拟中华水韭野外生长的条件下移栽幼苗,其成活率非常低,不足1%.研究表明,偏低的幼苗成活率可能是中华水韭居群规模难以扩大的主要原因之一.

The study was done to understand the first protected wild and critically endangered aquatic fern in China-Isoetes sinensis(Isoetaceae) and to provide more detailed proof about its artificial propagation and emigrate protection.The germination rate(GR) of megaspores were statisticed through aseptic culture of spores for rapid propagation in I.sinensis,Effects of temperature on the megaspores′ germination rate were compared and discussed.Result shows the highest germination rate is about 47%,after megaspores ...