基于USB的卵母细胞电压钳同步数据采集和分析软件设计

Software Design for USB-Based Oocyte Two-Electrode Voltage Clamp Amplifier

介绍了基于USB接口的卵母细胞电压钳系统的数据采集分析系统,应用Delphi语言开发了系统的人机操作实验界面,探讨了电压钳的USB同步传输、数据采集和和显示的实现方法.在细胞模型测试中,电压钳放大器的跨膜电流分辨达到了0.01nA,噪声测试低于10pA.在非洲爪蟾卵母细胞表达Kv4.2钾通道实验中,记录跨膜电流波形清晰,数据分析可靠.与国外同类仪器相比:该系统操作简单,价格低,数据采集和分析方便,适用于卵母细胞表达实验.

This paper presents the USB-based data acquisition and analysis software for oocyte two-electrode voltage clamp amplifier voltage clamp.On Delphi plarform,the experiment software was developed for data acquisition of oocyte membrane currents.The Man-Machine interface was discussed and optimized to be friendly manipulated.The experiment on cell model and expressed Kv4.2 potassium channel on xenopus laevis ooeyte was carried out,the responsible recording currents traces are clear,smooth and low-noise,the reso...