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靶向Livin基因siRNA载体的构建和沉默效应观察 被引量：3

Construction of siRNA vector targeting human Livin gene and observation of its silence efficiency
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摘要 目的:构建针对人Livin基因的小干扰RNA(siRNA)表达载体,并观察该载体对食管癌细胞Livin基因表达的沉默效应。方法:依据siRNA设计原则,分析Livin mRNA序列,设计并合成2个Livin靶向的发夹状siRNA靶序列,退火后分别连接入pSUPER-neo载体,转化扩增后进行序列测定。用脂质体包裹法将重组质粒转染人食管癌细胞EC9706,采用RT-PCR检测转染细胞Livin mRNA的表达。结果:经过酶切鉴定与测序,构建的靶向Livinα和Livinβ基因的siRNA载体,结果正确;RT-PCR检测显示,转染细胞Livin mRNA的表达水平降低。结论:成功构建沉默细胞Livin基因表达的siRNA载体。 Aim:To construct the siRNA expression vector targeted human Livin gene and observe its effciency.Methods:Specific short chain oligonucletide was designed according to the mRNA sequence,the double chain DNA sequenc was gained through annealing after chemosynthesis and was inserted to pSUPER-neo vector.The recombinant expression vector was transfected into human esophageal carcinoma cell line EC9706 by Liposome.The Livin mRNA in transfected cells were determined by RT-PCR.Results:Identificated by enzyme cutti...
作者 何群力 赵国新 苗立群 孙翔 王永玲 张明智
机构地区 新乡医学院微生物学教研室 河南省医学科学研究所 宝鸡市第二人民医院检验科 新乡医学院病理生理学教研室 郑州大学第一附属医院肿瘤科
出处 《郑州大学学报（医学版）》 CAS 北大核心 2007年第4期617-620,共4页 Journal of Zhengzhou University(Medical Sciences)
基金 教育部"十五"211工程重点学科建设项目
关键词 RNA干扰 LIVIN 载体 RNA interference Livin vector
分类号 R735.1 [医药卫生—肿瘤]
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参考文献6

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二级参考文献9

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共引文献12

同被引文献23

  • 1陈尚武,马涧泉.PCR产物克隆方法的新进展[J].生物技术,1995,5(4):1-4. 被引量：3
  • 2杜广营,朱乃硕.利用同序异尾限制性内切酶快速克隆多基因片段的新方法[J].生物化学与生物物理进展,2006,33(6):584-588. 被引量：10
  • 3赵国新,徐慧丹,赵新合,冯龙,卫艳萍,胡军.针对Cox-2基因siRNA载体构建和沉默效应[J].河南科技大学学报（医学版）,2007,25(1):1-4. 被引量：2
  • 4欧阳平,李玉,严红,马立新.一种载体构建的新方法:一步克隆法[J].湖北大学学报（自然科学版）,2007,29(2):178-181. 被引量：13
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  • 8Colnaghi R, Connell CM, Barrett RM, et al. Separating the antiapoptotie and mitotic roles of survivin [J]. J Biol Chem, 2006, 281 (44): 33450-33456.
  • 9Invernizzi R, Travaglino E, Benatti C, et al. Survivin expression, apoptosis and proliferation in chronic myelomonoeytic leukemia [J]. Eur J Haematol, 2006, 76 (6): 494-501.
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引证文献3

二级引证文献6

郑州大学学报（医学版）
2007年 第4期

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