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SSEA-1、SSEA-3、Oct-4及hTERT在人胚胎生殖细胞中的表达 被引量：2

The expression of SSEA-1,SSEA-3,Oct-4 and hTERT in human embryonic germ cells
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摘要 分离5~9周人胚胎生殖腺嵴和背侧肠系膜,采用组织块法进行体外培养,免疫细胞化学染色法检测SSEA-1、SSEA-3、Oct-4及hTERT在人胚胎生殖细胞中的表达,以证实利用组织块体外培养所获得的细胞为未分化的人胚胎生殖细胞。结果表明:组织块体外培养得到的细胞或细胞集落均阳性表达SSEA-1、SSEA-3、Oct-4及hTERT,说明组织块体外培养获得的细胞为未分化的人胚胎生殖细胞。 Human embryonic genital ridges,dorsal mesenteries(5-9 weeks postfertilization) were cultured through tissue culturing and then use the immunocytochemistrical staining to observe the expression of SSEA-1,SSEA-3,Oct-4,and hTERT on the cultivated cells to show these cells cultured through tissue culturing were undifferentiated human embryonic germ cells.Both the individual cell,and the cluster of cells which cultured in vitro through tissue culturing are SSEA-1,SSEA-3,Oct-4,and hTERT positive,so it shows that ...
作者 李芳 陈永珍 陈谦 朱旻 张苏
机构地区 苏州大学医学院
出处 《扬州大学学报（农业与生命科学版）》 CAS CSCD 北大核心 2007年第4期21-24,共4页 Journal of Yangzhou University：Agricultural and Life Science Edition
基金 苏州市社会发展和三药基金资助项目(N3134507)
关键词 人胚胎生殖细胞 SSEA-1 SSEA-3 OCT-4 HTERT 表达 human embryonic germ cells SSEA-1 SSEA-3 Oct-4 hTERT expression
分类号 R321 [医药卫生—人体解剖和组织胚胎学]
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参考文献8

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二级参考文献10

共引文献28

同被引文献35

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扬州大学学报（农业与生命科学版）
2007年 第4期

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