磷酸二酯酶4D基因三个多态性与缺血性卒中的关系

Three polymorphisms in phosphodiesterase 4D gene and their association with ischemic stroke

目的:探讨磷酸二酯酶4D(PDE4D)基因与缺血性卒中(IS)的关系。方法选取121例缺血性卒中患者和130例正常对照,应用等位基因特异性聚合酶链反应(ASPCR)技术筛查PDE4D基因的单碱基多态性(SNP)45和SNP41,应用聚合酶链反应(PCR)结合聚丙烯酰胺凝胶电泳技术检测微卫星标记AC008818-1。对资料编码后输入SAS6.12统计软件进行分析。结果缺血性卒中患者组中发现一例SNP45和SNP41的双杂合状态,病例组和对照组SNP45、SNP41、AC008818-1多态性之间的差异均无统计学意义。结论本组人群中PDE4D基因SNP45、SNP41和AC008818-1多态性与缺血性卒中无关。

Objective To investigate the relationship between phosphodiesterase 4D(PDE4D)gene and ischemic stroke.Methods All 121 ischemic stroke patients and 130 controls were recruited in our study,their single nucleotide polymorphism(SNP)45 and SNP41 of PDE4D gene were detected by alleles specific polymerase chain reaction(ASPCR)and their microsatellite AC008818-1 were deteced by polyacrylamide gel electrophoresis following polymerase chain reaction.Data were coded and entered in SAS6.12 were analyzed.Results Double...