柠檬酸与乙酸酐混合溶液中分光光度法测定盐酸西布曲明

Spectrophotometric Determination of Sibutramine Hydrochloride in Citric Acid-Acetic Anhydride Mixed Solution

柠檬酸和乙酸酐混合溶液与盐酸西布曲明在80℃的水浴中加热30 min,反应后使溶液呈紫黑色,在330 nm波长处吸收最大,据此建立测定盐酸西布曲明的分光光度法。盐酸西布曲明的质量浓度在0.17~18.5 mg.L-1范围内遵守比耳定律,相关系数为0.998 9,表观摩尔吸光率为8.16×104L.mol-1.cm-1,检出限为61.5μg.L-1。用于曲美胶囊中盐酸西布曲明的测定,测定结果与高效液相色谱法结果相符,其相对标准偏差(n=6)值均小于0.5%。

It was found that when sibutramine hydrochloride(abbreviated as SBTM) was reacted with the mixed solution of citric acid and acetic anhydride at 80 ℃ in water bath,a purple black solution was obtained.The absorption maxima of the solution was found at 330 nm,and Beer′s law was obeyed in the range from 0.17 mg·L-1 to 18.5 mg·L-1(r=0.998 9),with its detection limit of 61.5 μg·L-1.Molar absorptivity of the colored solution was found to be 8.16×104L·mol-1·cm-1.The proposed method has been applied to the analysi...