肺癌患者Th1/Th2细胞因子的免疫反应状态变化及临床意义

The Reaction State and Clinical Signficance of TH1/TH2 Cytokines in Lung Cancer

目的:分析肺癌患者外周血分泌不同细胞因子的T细胞数以及血清中6种细胞因子的水平,为肿瘤的免疫治疗提供实验依据。方法:先用刺激物刺激外周血T细胞,以增加细胞内细胞因子的表达,继用流式细胞仪分析特异性细胞因子表达水平。同时用酶联免疫吸附试验(ELISA)检测血清中相应的细胞因子量。结果:肺癌患者Th1型细胞因子干扰素-γ(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-12(IL-12)表达水平较正常对照组显著降低,Th2型细胞因子白细胞介素-4(IL-4)和白细胞介素-10(IL-10)表达水平较正常对照组升高,差异有显著性。肿瘤坏死因子-a(TNF-a)表达水平较正常对照组升高,差异有显著性意义。结论:肺癌患者体内Th2型细胞因子模式占优势状态,这可能是肿瘤细胞发生免疫逃逸的原因之一。

Objective To analysed the amount of Tlymphocytessecreting different cytokines by peripheral blood and the serum level of those cytokines in the patients with lung cancer. In order to find out the laboratory evidence of tumor immunotherapy.Methods The peripheral blood lymphocytes were stimulated by Stimulator in order to enhance expression of cytokines . Those cytokines were detected by flow cytometry and enzyme linbed immunosorbent assay(ELISA). Results The level of Th1 type cytokines such as interferon-γ(I...