^(60)Co-γ射线辐射紫花苜蓿种子的细胞生物学效应研究

Cytological effects of ^(60)Coγ-rays irradiation on seeds of Medicago sative L.

分别利用0,1000,1500和2000Gy60Coγ射线处理3个品种的紫花苜蓿种子,对同一剂量不同品种或同一品种不同剂量间辐射情况进行比较,研究苜蓿根尖细胞的遗传学效应.结果表明,γ射线辐射苜蓿种子可以抑制根尖细胞有丝分裂,并诱发根尖细胞产生单微核、双微核、多微核、小核、染色体断片、染色体粘连、单桥、双桥、多桥、游离染色体、落后染色体等多种畸变.同一辐射剂量下,龙牧803苜蓿对γ射线辐射敏感性最强.在0～2000Gy范围内,随剂量的增加,各种畸变率不断提高,至2000Gy辐射剂量时,各种畸变率达到最高.

Seeds of three genotypes Medicago sative.L were irradiated by 0Gy,1000Gy,1500Gy,2000Gy 60Coγ-rays,then we compare irradiated states of two different conditions,one is the same dosage of different genotype,the other is the same genotype of different dosage,and the genetics effects on root tip cell in Medicago sative L were studied.The results showed that the irradiation of γ-rays inhibited mitoses and produced various aberrations,such as single micronucleus,double micronucleus,multi-micronucleus,small micron...