软脉灵对动脉粥样硬化鹌鹑血管组织MMP-2、MMP-9活性及其基因表达的影响

Effect of Ruanmailing on MMP-2,MMP-9 of Aortic Vessels in Quail Atherosclerosis Model

为探讨软脉灵抗动脉粥样硬化的作用机理,采用高脂餐喂饲鹌鹑复制动脉粥样硬化模型,同时给予软脉灵,16 w后,取主动脉,制病理切片,明胶酶谱法测定血管中基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)的活性,RT-PCR半定量法测定血管组织中MMP-2、MMP-9的基因表达。结果表明,动物喂饲高脂饲料16 w后,主动脉内膜厚度显著增加,动脉粥样硬化斑块形成,动脉血管组织中MMP-2、MMP-9的活性及基因表达均增加。给予软脉灵干预治疗后MMP-2、MMP-9的活性和基因表达都下降。表明软脉灵可能通过稳定动脉粥样硬化斑块,防止动脉粥样硬化斑块破裂而达到防治冠脉综合征(冠心病、心绞痛等)。

To explore the anti-atherosclerosis mechanism of Ruanmailing,the atherosclerosis model was made by feeding high fat diet,and the Japanese male quails were given Ruanmailing at the same time.After 16 weeks,the quails were killed and the aortae were collected to examine the content of MMP-2,MMP-9.The results showed that the thickness of endomembrane in model animals increased,the atherosclerostic plaque formed,and the content of MMP-2,MMP-9 increased,but the content of MMP-2,MMP-9 decreased after giving Ruanmailing for 16 weeks.It can be concluded that stabilizing the atherosclerosticplaque and preventing the rupture of atherosclerosticplaque were probably was the mechanism of preventing and curing coronary artery syndrome(coronary heart disease,angina pectoris) of Ruanmailing.