健康人胃液和肠液中砷等微量元素的原子荧光光谱快速测定法

Rapid Determination of Arsenic, Mercury and Selenium in Human Gastric and Intestinal Juice by Atomic Fluorescence Spectrometry

目的探讨健康人群的胃、肠液中砷、汞、硒的原子荧光光谱测定法。方法样品中痕量的砷、汞、硒与强还原剂硼氢化钾反应生成砷、硒的气态氢化物和汞蒸气与基体成分分离。结果砷、汞、硒的线性范围分别为5.00～80.00ng/ml,0.10～4.00ng/ml,0.50～5.00ng/ml,RSD均小于6.0%,检出限分别为0.20、0.01、0.33ng/ml,加标回收率分别为98.6%～104.4%,92.0%～104.0%,98.5～100.5%。结论该法具有简便、快速、准确等优点,结果令人满意。

Objective To establish a new atomic fluorescence spectrometry for the determination of As,Hg and Se in human gastric and intestinal juice. Methods The KBH4 solution was used as a reducing agent to generate hydrides of As,Se and metallic vapour of Hg from samples. Results The lineal range of the method was 5.00-80.00 ng/ml for As, 0.10-4.00 ng/ml for Hg, 0.50-5.00 ng/ml for Se respectively. The RSD was less than 6.0%. For As, Hg and Se, the limits of detection were 0.20, 0.01, 0.33 ng/ml respectively, the recovery rates were 98.6%-104.4%, 92.0%-104.0%, 98.5%-100.5% respectively. Conclusion The method is simple, rapid and accurate with satisfactory results.