GSTT1和GSTM1基因多态性与砷中毒易感性的相关性研究

GSTM1,GSTT1 Gene Polymorphisms and Susceptibility to Chronic Arsenic Poisoning

目的探讨GSTT1、GSTM1基因多态性与砷中毒易感性的相关性。方法选择某工业性砷污染区常住居民中的174名砷中毒患者(病例组)和92名正常人(对照组)作为研究对象。应用多重PCR技术检测GSTM1和GSTT1基因多态性。结果病例组GSTM1(+)的百分比(39.66%)高于对照组(27.17%),两组间差异有统计学意义(P<0.05);GSTM1(+)人群患砷中毒的危险性是GSTM1(-)的人群的1.7611倍(95%CI为1.0154～3.0546)。病例组GSTT1(+)的百分比(60.34%)高于对照组(45.65%),两组差异有统计学意义(P<0.05);GSTT1(+)人群患砷中毒的危险性是GSTT1(-)的人群的1.8116倍(95%CI为1.0874～3.0182)。GSTM1和GSTT1同时为非空白基因型人群患砷中毒的危险性是GSTM1和GSTT1同时为空白基因型人群的3.0833倍(95%CI为1.4148～6.7196)。结论GSTT1和GSTM1基因多态性与砷中毒的发生可能有关,在相同砷暴露环境下,GSTT1和GSTM1空白基因型个体患砷中毒的危险性可能更低。

Objective To analyze the relationship between genetic polymorphisms of glutathione-S-transferase (GST) M1 and T1 and the susceptibility to chronic arsenic poisoning. Methods 174 chronic arsenic poisoning sufferers and 92 normal controls in an arsenic polluted village were randomly selected as the subjects. The genetic polymorphisms of GSTM1 and T1 were detected by multiple PCR method. Results The frequency of GSTM1 positive genotype in the case group and control group was 39.66% and 27.17% respectively, the individuals with GSTM1 positive genotype had significantly higher risk of arsenic-induced skin lesions(OR=1.7611, 95%CI: 1.0154-3.0546). The frequency of GSTT1 positive genotype in the case group was 60.34%, which was significantly higher than that in the control group (45.65%), individuals with GSTT1 positive genotype had significantly higher risk of arsenic-induced skin lesions(OR=1.811 6, 95%CI: 1.087 4-3.018 2). The risk of arsenic-induced skin lesions in the individuals with both GSTM1 and GSTT1 positive genotype was 3 times as much as that in the individuals with both GSTM1 and GSTT1 null genotype. Conclusion The genotype of GSTT1 and GSTM1 may relate to the chronic arsenic poisoning. In the same arsenic exposure, the individuals with the null genotypes of GSTT1 and GSTM1 may have lower susceptibility to chronic arsenic poisoning.