纳米红色元素硒对四氯化碳致大鼠肝损伤的影响

Effects of Nano Red Elemental Selenium on Expression of bax and p53 Protein in Experimental Liver Injure of Rats

目的探讨纳米红色元素硒在CCl4诱发大鼠实验性肝损伤中的干预作用。方法将清洁级Wistar大鼠按体重随机分为阴性对照组、阳性对照组、纳米红色元素硒对照组、纳米红色元素硒高、中、低剂量组,分别灌胃给予生理盐水、生理盐水、纳米红色元素硒(0.2、0.2、0.1、0.05mg/kg)。8d后,阴性对照组和纳米红色元素硒对照组一次性腹腔注射植物油(3ml/kg),阳性对照组和纳米红色元素硒高、中、低剂量组均一次性腹腔注射5.18mol/L四氯化碳(3ml/kg)。24h后,处死大鼠,称量体重、肝脏重量,计算肝脏系数。测定大鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活力,采用HE染色法检测肝组织病理情况,采用免疫组织化学法检测大鼠肝组织中bax、p53基因蛋白表达。结果阴性对照组和纳米红色元素硒对照组肝组织正常;阳性对照组、纳米红色元素硒高、中、低剂量组肝脏体积增大,颜色灰暗,并有腹水,肝组织小叶内出现气球样变和脂肪变,并有大量中性粒细胞浸润。纳米红色元素硒对照组与阴性对照组肝脏系数、AST、ALT活力、bax、p53基因蛋白表达的积分光密度(IOD)值和阳性面积间,差异均无统计学意义(P>0.05)。阳性对照组、纳米红色元素硒高、中、低剂量组肝脏系数、AST、ALT活力、bax、p53基因蛋白表达的IOD值和阳性面积高于阴性对照组、纳米红色元素硒对照组,差异均有统计学意义(P<0.01);且纳米红色元素硒对照组<低剂量组<中剂量组<高剂量组。结论在本次实验剂量下,纳米红色元素硒对大鼠肝脏无损伤作用,对CCl4诱导的急性实验性肝损伤无明显保护作用。

Objective To explore the interferential role of nano red elemental selenium in experimental liver injure of rats induced by carbon tetrachloride. Methods 60 Wistar rats were randomly divided into six groups. Interferential experiment groups were treated with nano red elemental selenium at 0.2、0.1、0.05 mg/kg i.g. Experimental control group was treated with nano red elemental selenium 0.2 mg/kg i.g. 0.9% sodium chloride solution were used both the positive and normal control group. After 8 days of treatment, the normal control group and experimental control group were treated with bean oil (3 ml/kg·bw) i.p once. The positive group and interferential experiment groups were treated with 5.18 mol/L CCl4(3 ml/kg·bw) i.p once. All rats were put to death after 24 h. The activity of alanine aminotransferase (ALT) and aspartatetrans aminase (AST) in the serum were determined. The histopathological examination was conducted. bax and p53 protein in the livers were determined with immunohistochemical method. Results Histopathological results showed that the liver tissues were normal both in normal control group and experimental control group. The changes among the positive group and interferential experimental groups were that the livers increased in volume, color gloomed, ascetic fluid appeared in peritoneal cavity, and then ballooning degeneration, fatty degeneration and inflammation cells infiltration were found in the hepatic lobules. No significantly difference was seen in the activity of ALT and AST in the serum and the expression of bax, p53 between the experimental control group and normal control group (P>0.05), the same results were seen in the interferential experiment groups and the positive group. There were no alternating effects between nano red elemental selenium and CCl4. Conclusion In the present paper, nano red elemental selenium does not show an adverse effect on the liver of rats and a protective effect on liver injury induced by CCl4.