鱼藤酮对大鼠中脑原代培养细胞的毒性

Adverse Effects of Rotenone on Ventral Mesencephalic Neurocytes of Rats in Culture

目的研究植物杀虫剂鱼藤酮(Rotenone)对大鼠中脑原代培养细胞的毒性作用。方法采用大鼠胚胎中脑原代细胞培养法,用0.01、0.10、1.00μmol/L鱼藤酮对体外培养中脑原代培养细胞染毒24h,通过免疫细胞化学的方法观察神经细胞的存活数及形态学改变。结果与对照(0μmol/L)组比较,0.10和1.00μmol/L鱼藤酮染毒组多巴胺神经元数目明显降低,差异有统计学意义(P<0.05,P<0.01);1.00μmol/L鱼藤酮染毒组星型胶质细胞数目明显下降,差异有统计学意义(P<0.05)。随着鱼藤酮染毒浓度的升高,存活的多巴胺(DA)能神经元体积变小,突起减少、变短或断裂。结论多巴胺神经元对鱼藤酮的毒性作用更敏感。

Objective To study the neurotoxicity induced by rotenone to the primary cultured ventral mesencephalic dopaminergic neurons. Methods SD rat ventral mesencephalic neurocytes were cultured and identified by tyrosine hydroxylase (TH) and glial fibrillary acid protein (GFAP). Immunohistochemistry and morphological observation were used to evaluate the toxicity of rotenone to the dopaminergic neurons and astroglia. Results After 24 h of exposure to rotenone at 0.10 and 1.00 μmol/L, the number of surviving dopaminergic neurons significantly decreased. The number of surviving astrocytes significantly decreased treated with 1.00 μmol/L. Exposed to 0.10 μmol/L rotenone, the morphological change of the dopaminergic neurons was seen, the area of cell body decreased significantly. Conclusion The dopaminergic neurons is more susceptible to the neurotoxicity of rotenone.