所谓肺硬化性血管瘤组织中两种主要细胞的基因表达差异及意义

Differential gene expressions of polygonal cells and cuboidal cells in so-called pulmonary sclerosing hemangioma

背景与目的所谓肺硬化性血管瘤(so-called pulmonary scleraosing hemangioma,PSH)组织中两种形态不同的主要细胞--间质内多角形细胞和表面立方细胞的某些基因的表达是否相同尚不清楚。揭示他们的基因表达异同点,对于理解两种细胞的分化状态、组织发生以及对该肿瘤进行诊断和鉴别诊断具有重要的意义。方法应用激光捕获显微切割技术(laser capture microdissection,LCM)获取PSH组织中多角形细胞和表面立方细胞,分别提取总RNA,应用RT-PCR方法分别检测形态上完全不同的两种细胞的角蛋白(cytokeratin,CK)、上皮膜抗原(epitheliem membrane antigen,EMA)、波形蛋白(vi mentin,VT)、表面蛋白(surfactant protein B,SP-B)、甲状腺转录因子1(thyroid transcription factor-1,TTF-1)、突触素(synaptophys-in,Syn)及嗜铬素(chromogranin A,ChA)的mRNA表达。结果表面立方细胞具有明确的CK、EMA和SP-B的基因表达,间质内多角形细胞则有明确的VT、Syn和弱的EMA表达,而TTF-1在两者中都有表达,ChA在二者中都无表达。结论PSH组织中的多角形细胞和表面立方细胞在形态表型上的差异可能是由于所处不同的分化状态所致,表面立方细胞趋向肺泡Ⅱ型细胞分化,而间质内的多角形细胞则具有多向分化的潜能。

Background and objective The gene expressions of the two primary cell types with different morphological phenotypes,namely,cuboidal cells and polygonal cells in so-called pulmonary sclerosing hemangioma(PSH) were still unclear now.Disclosing the different gene expression of the two cell types will provide the basis for the study of histogenesis of PSH tissue and be helpful for differential diagnosis.Methods Polygonal and cuboidal cells were obtained from PSH tissue using a laser capture microdissection(LCM) technique.Total RNA was extracted and mRNA levels of cytokeratin(CK),epithelial membrane antigen(EMA),vimentin(VT),surfactant protein B(SP-B),thyroid transcription factor-1(TTF-1),synaptophysin(Syn),and chromogranin A(Ch-A) were analyzed by RT-PCR.Results The CK,EMA and SP-B genes were clearly expressed in cuboidal cells,while the VT and Syn genes were clearly expressed and the EMA gene was weakly expressed in polygonal cells.TTF-1 was expressed in both cell types,while neither cell type expressed ChA.Conclusion The differences in morphological phenotype might result from differences in the state of differentiation,cuboidal cells may differentiate into type Ⅱ pneumocytes,while polygonal cells in stroma possess the multipotency differentiation.