Pokemon蛋白在非小细胞肺癌中的表达及临床意义

Expression and clinical significance of Pokemon in non-small cell lung cancer

背景与目的原癌基因Pokemon是ARF特异性转录抑制剂,通过ARF-P53途径调节细胞生长和分化。Pokemon是多种肿瘤抑制基因和原癌基因的上游基因,因此有望成为重要的肿瘤监视靶点。本研究旨在探讨Pokemon基因在非小细胞肺癌(NSCLC)中表达的临床意义及其对预后的影响。方法应用SP免疫组化方法检测Pokemon蛋白在92例NSCLC肿瘤组织及20例癌旁组织中的表达,分析其与NSCLC临床病理学特征及预后的关系。结果Pokemon在癌旁组织中不表达,92例肺癌组织中66例呈阳性表达(占71.7%)。Pokemon表达与肺癌患者TNM分期有密切关系(P=0.011)。Pokemon阴性表达者生存率显著高于Pokemon阳性表达者(P=0.0015)。Pokemon表达是影响患者预后的独立因素。结论NSCLC中存在Pokemon的表达,它有望作为NSCLC新的诊断标志。Pokemon高表达可能预示NSCLC患者预后较差。

Background and objective Proto-oncogene Pokemon is the special transcription inhibitor of ARF,which can regulate cell growth and differentiation by ARF-P53 path.It may be the important monitoring target of tumor because of being upstream region of many tumor suppressor genes and proto-oncogenes.The aim of this study is to explore the clinical significance of Pokemon gene in non-small cell lung cancer(NSCLC).Methods Immunohistochemistry was applied to detect the expression of Pokemon protein in 92 cases of NSCLC and 20 cases of paracancerous lung tissues.Correlation between abnormal expression of Pokemon with pathologic characteristics and prognosis of NSCLC was analyzed.Results Pokemon was not expressed in paracancerous lung tissues and was found in 66 of 92(71.7%) cases of lung cancer tissues.Expression of Pokemon was closely related to TNM stages(P=0.011).Survival rate of patients with negative Pokemon expression was significantly higher than that of those with positive Pokemon expression(P=0.0015).Pokemon expression was demonstrated as independent prognostic factor of NSCLC.Conclusion Pokemon is expressed in NSCLC and it may be identified as a new diagnostic marker.High expression of Pokemon may indicate poor prognosis of patients with NSCLC.