TP-ELISA法在梅毒临床诊断中的应用

Application of TP-ELISA in the clinical diagnosis of syphilis

目的:评价梅毒螺旋体酶联免疫吸附试验(Enzyme-linked immunosorbent assay,TP-ELISA)检测梅毒螺旋体抗体IgG/IgM的临床意义。方法:收集经临床确诊的各期梅毒血清80份,自身免疫性疾病并排除梅毒的患者血清样本40份,以及100份献血员血清样本。全部血清标本分别进行RPR、TPPA和TP-ELISA试验,检测梅毒螺旋体抗体。结果:TP-ELSIA在梅毒患者组中的阳性率为100%(80//80),确证试验TPPA的阳性率为98.8%(79/80),两种方法的检测阳性率无统计学差异(P>0.05)。TP-ELSIA检测阳性率明显高于初筛试验RPR的检测阳性率87.5%(70/80)。在自身免疫性疾病组RPR试验出现假阳性,其他两种方法无假阳性出现。结论:TP-ELISA检测梅毒螺旋体特异性IgG/IgM抗体具有较高的敏感性和特异性,适用于临床大批量样本初筛和确诊,TP-ELISA试验阳性标本建议进一步作RPR试验。

Objective:To evaluate the clinical significance of Enzyme-linked immunosorbent assay(ELISA)for detection of IgG/IgM against treponema pallidum.Methods:The serum samples collected from 80 syphilitic patients who had been diagnosed correctly by clinic、40 autoimmune diseases patients excluding syphilis and 100 healthy blood-donors,then they were tested for antibody against treponema pallidum by RPR、TPPA and TP-ELISA methods respectively.Results:The positive rates of TP-ELISA、TPPA and RPR for detection of antibody against treponema pallidum were 100%(80//80)、98.8%(79/80)and 87.5%(70/80)respectively.There was no statistical difference between TPPA and TP-ELISA for diagnose of syphilis(P>0.05).The false positive rate of RPR is 5.0%(2/40)in autoimmune diseases group.It didn't appear in TPPA and TP-ELISA methods.Conclusion:TP-ELISA in diagnosing for syphilis can be taken as a screening and confirmatory test for large number of clinical samples.It has both high sensitivity and high specificity in diagnosis of syphilis.The positive samples tested by TP-ELISA should also be tested by RPR.