摘要
以大豆品种合丰25的体细胞胚为受体,以Bt基因为目的基因,应用基因枪法进行了遗传转化,经体细胞胚萌发、生根及壮苗培养和卡那霉素选择培养,获得了完整的抗性再生苗,经PCR、PCR-Southern和点杂交分析鉴定,初步证明外源基因Bt已整合到大豆的基因组中。
The Somatic embryo of soybean Hefeng 25 were transformed by microprojectile bombardment.The resistant plants to kanamycin were obtained,two positive plants were obtained among them by PCR,and the positive plantlets were further analyzed by dot blotting and PCR-Southern blotting.The results demonstrated that the target Bt gene had been transformed into the soybean genome.
出处
《大豆科学》
CAS
CSCD
北大核心
2007年第2期140-143,共4页
Soybean Science
基金
"国家植物转基因技术研究开发与中试基地建设"专项课题(J99-B-001)
