脂质体用大豆卵磷脂的制备及质量评价

PREPARATION AND QUALITY EVALUATION OF SOYBEAN PHOSPHATIDYLCHOLINE AS LIPOSOMAL MATERIAL

以大豆磷脂为原料,用溶剂-超临界CO2萃取法制备脂质体用高纯度大豆卵磷脂,并对其质量进行评价。结果表明大豆卵磷脂的含量是94.98%,丙酮不溶物的含量是99.0%,酸值是20.6KOH mg/g,干燥失重是0.63%,己烷不溶物的含量0.18%,砷盐的含量是2.1×10-6%,重金属的含量是2.0×10-5%。以精制的大豆磷脂为原料制备的脂质体,在透射电镜下呈圆球状。粒径为164 nm,Zeta电位为-28.50 mv,包封率是32.43%。

High-purity soybean phosphatidylcholines(SPC) as liposomal material was prepared from soybean phospholipids with a method combined super critical carbon dioxide extraction and solvent extraction.The results of the quality evaluation showed that the SPC was separated with94.98% purity,the content of aceton-insoluble matter,hexane-insoluble matter,heavy metals and arsenic were respectively 99%,0.18%,2.0 10-5% and 2.1 10-6%,the acid value was 20.6 mg of potassium hydroxide,loss on drying of SPC was 0.63%.The liposome made with SPC showed spherical shape,its main particles was164 nm,Zeta potential was-28.50 mv,and the entrapment efficiency was 32.43%.