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转座子标签法及其在大豆功能基因组研究中的应用前景 被引量:1

TRANSPOSON TAGGING AND ITS POTENTIAL USE IN FUNCTIONAL GENOMICS STUDY OF SOYBEAN
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摘要 转座子标签法是克隆未知基因的有效方法之一。本文介绍了转座子标签法的基本原理、步骤、方法及其在植物基因克隆中的应用情况,并对该方法在大豆突变体库构建和功能基因组研究中的应用前景进行了讨论。 Transposon tagging is a powerful molecular tool for isolating genes with unknown functions.This paper reviewed the fundamental,procedure and method of transposon tagging,as well as the recent advances of transposon uses in gene cloning.The application of transposon tagging in the mutant pool establishment and functional genomics study of soybean was also discussed and prospected.
出处 《大豆科学》 CAS CSCD 北大核心 2007年第2期254-258,共5页 Soybean Science
基金 国家重点基础研究发展计划(973)项目(2002CB111301-3) 北京市自然科学基金(5042019) 农业部作物遗传育种重点开放实验室资助
关键词 转座子标签法 大豆 基因克隆 双因子标签法 Transposon tagging Soybean Gene cloning Two element transposon tagging system
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参考文献27

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二级参考文献3

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