紫外分光光度法测定大豆中异黄酮的含量

DETERMINATION OF SOYBEAN ISOFLAVONES WITH ULTRA-VIOLET SPECTROPHOTOMETRY

建立一种测定大豆中异黄酮类成分含量的紫外分光光度法分析方法。以染料木素为对照品,利用染料木素与氢氧化钠产生反应,在271 nm波长处有最大吸收峰,用紫外分光光度法测定大豆中总异黄酮的含量。大豆中总异黄酮的含量为4.784 mg/g,加样回收率为100.9%,相对标准偏差为2.0%。方法简便、准确、重现性好,可作为检查大豆中异黄酮的含量的一种手段,适用于大豆及其保健品的日常分析和质量控制。

A UV spectrophotometry used for determination of soybean isoflavones was established.Genistein was selected as the reference substance.Based on the reaction of genistein and NaOH,which has the maximum absorption in 271 nm wavelength,ultra-violet spectrophotometry method was used for determination of isoflavones in Glycine max(L.) Merr..The total content of isoflavones in soybean extracts was 4.784 mg/g with mean recovery 100.9%,and the RSD was 2.0%.The method is suitable for the routine analysis and quality control of the soybean and function food with easy-to-handle,good accuracy and reproducibility.