摘要
应用组织培养技术及MTT法,对鹅源H5N1型AIV感染雏鸡胸腺、脾脏T细胞增殖功能及IL-2诱生活性进行检测,结果发现:雏鸡感染AIV后,免疫器官T细胞增殖功能和IL-2诱生活性在感染初期较对照雏鸡明显降低,后期有所恢复,表明AIV感染雏鸡细胞免疫受到抑制,中枢与外周免疫器官分子免疫调节功能障碍。7和21日龄AIV感染雏鸡均发病,但21日龄感染雏鸡上述指标的下降时间较7日龄感染雏鸡短且相对滞后,死亡率也较低,表明21日龄雏鸡免疫能力较强,具有一定的抗AIV感染能力。
In this study,cell culturing and MTT chromometry were used to detect the proliferation of T lymphocytes and IL-2 inductive activity in thymus and spleen of chickens infected with avian influenza virus(AIV) separated from goose. The results showed that above indexes of infected chickens were decreased remarkably in the initial stage of infection compared with that of controls and then recovered,which revealed that cell-mediated immunity of infected chickens was inhibited and molecular immune regulation of cytokines in central and peripheral immune organs was disordered. Two group of infected chickens were all invalid,but the depressing time of T lymphocytes proliferation and IL-2 inductive activity was shorter and later of chickens infected at 21 days old than that of 7 days old. The results indicated the chickens of 21 days old had certain resistibility to AIV.
出处
《中国家禽》
北大核心
2007年第16期8-10,20,共4页
China Poultry
基金
国家自然科学基金资助项目(30371066)
