雏鸭人工感染黄曲霉毒素及其毒素含量的动态变化

Experimental Acute Poisoning of Aflatoxin and the Content Changes in Duckling

通过7日龄樱桃谷鸭饲喂黄曲霉毒素(AFB1)含量为150bbp的全价饲料,复制雏鸭感染黄曲霉毒素急性中毒病例,观察中毒鸭的临床症状;于6、12、24、48、72、96、120、144、168和192h分别剖杀2只,观察大体病理变化,并用ELISA试剂盒测定血液、肝脏中的AFB1含量。黄曲霉毒素急性中毒病例的临床症状主要为:生长停滞,跛行,运动失调,死前呈角弓反张等神经症状。剖检病理变化主要为:气囊混浊有黄色纤维素样渗出物,肝脏肿大呈土黄色,质地变脆并有出血点。饲喂黄曲霉毒素饲料6h后即可从血液、肝脏中检出AFB1,随着饲喂黄曲霉毒素饲料时间的延长,血液、肝脏中AFB1的含量逐渐增高,7d时肝脏和血清中的AFB1分别高达53.4ng/g和35.68ng/g。

Seven-day-old Cherry Valley ducklings were fed with the diet containing 150 bbp aflatoxin(AFB1) for observing clinical symptom of acute aflatoxin poisoning. Two ducklings were dissected at 6,12,24,48,72,96,120,144,168,192 hours after feeding to study the macroscopic pathological changes,and the content of AFB1 in blood and liver was detected by ELISA. The clinical symptoms was mainly that growth stagnated,lamely movement disorders,opisthotonos before death and other neurological symptoms. Macroscopic characteristic was that much yellow fibrin exuded from air cell, liver was swollen. AFB1 in liver and blood could be detected after feeding 6 hours. With the feeding time prolonged,the amount of AFB1 in blood and liver increased constantly. The amount of AFB1 in liver and blood was 53.4 ng/g and 35.68 ng/g at 7 day after feeding.