高效液相色谱法测定碘昔兰注射液的含量

HPLC Determination of Ioxilan Injection

目的建立用HPLC法测定碘昔兰注射液含量的方法。方法采用IntersilNH2柱(250mm×4.6mm,5μm),以乙腈:水(87:13)为流动相,流速为1.0mL.min-1,检测波长为245nm,柱温为30℃。结果碘昔兰对照品在0.12～30μg范围内线性关系良好,回归方程为A=35.911C+0.7928,R2=0.9999。平均回收率为100.6%,RSD为0.60%。结论本方法简便快捷、结果准确,可用于碘昔兰注射液的含量测定。

OBJECTIVE To establish a HPLC method for determination of Ioxilan injection.METHODS Chromatographic conditions:column:Intersil NH2(250 mm×4.6 mm,5 μm),mobile phase:acetonitrile:water(87:13) with a flow rate of 1.0 mL·min-1,detector:UV detector at 245 nm,temperature:30℃.RESULTS The linear range was 0.12~30 μg,the regression equation was A=35.911C+0.7928,R2=0.9999,the mean recovery was 100.6% and RSD is 0.60%.CONCLUSION The HPLC method is simple,sensitive and reproducible for determination of Ioxilan injection.