爱宁的致突变作用研究

Evaluation of Erianin Mutagenicity in Vitro and in Vivo

目的检测爱宁的致突变性,以提供有关致突变的遗传毒性安全评价数据。方法设1.6,8,40,200和1000μg/皿5个剂量组,进行Am es试验;设0.3,1,3和9μg.mL-14个剂量组,进行仓鼠肺成纤维细胞染色体畸变试验;设15,100和250 mg.kg-13个剂量组,观察其对小鼠骨髓细胞微核的影响。结果Am es试验表明,爱宁在加和不加S9的条件下,对TA97,TA98,TA100和TA102菌株回复突变菌落结果为阴性。仓鼠肺成纤维细胞染色体畸变试验表明,爱宁对中国仓鼠肺成纤维细胞体外培养染色体无畸变作用。小鼠骨髓细胞微核试验表明,爱宁三个剂量组的微核出现率与阴性对照组比较无显著性差异。结论在本实验条件下,爱宁无致突变性。

OBJECTIVE To investigate the mutagenicity of Erianin,in order to asses it safety.METHODS The Ames test was set up using doses of 1.6,8,40,200 and 100 g/plate,the CHL test,at the dose of 0.3,1,3 and 9 g·m-1,bone marrow cell micronuclear test in mice,at the dose of 15,100 and 250 mg·kg-1. RESULTS The mutagenic activity of Erianin was not observed in TA97,TA98,TA100 or TA102 with or without S9 activation;In CHL test,the effect of chromosomal damage of Erianin was not found either in CHL cells;No significant difference in the micronuclear frequences were found in any of testing groups compared with negative control in mice.CONCLUSION Our study showed that Erianin had no mutagenicity.