磁性高分子微球固定化酶的制备及应用

Preparation and application of magnetic microsphere immobilized enzyme

利用磁性高分子微球通过化学反应固定化酶,可以借助外部磁场方便地分离回收固定化酶,将固定化酶放入磁场稳定的流动床反应器中还可以减少持续反应体系中的操作。简要地介绍了磁微球的制备方法,包括包埋法、分散聚合、乳液聚合和悬浮聚合,对磁性微球固定化酶的制备方法和原理进行了探讨,论述了磁性高分子微球固定化酶的特点及应用。

Magnetic polymer microspheres are characterized by potent magnetic susceptibility and reactive polymer surfaces with large specific surface area,on which functional molecules,such as enzyme,can be immobilized by different methods.With a powerful magnetic field,they can be moved and separated directly from the dispersion medium,and thus can be repeatedly reused.In addition,by applying an external magnetic field,they can be more easily stabilized in a fluidized-bed reactor.Different methods for preparing magnetic microspheres,including the magnetite coated with a polymer,emulsion polymerization,dispersion polymerization and suspension polymerization,were introduced in the paper.Further,the immobilization of enzymes using different functional groups was also reviewed regarding the characteristics and application of the immobilized enzyme.