摘要
Neuregulin plays an important role in heart structure and function.Research discovered that recombinant neuregulin could reduce the degree of damage on myocardial cells caused by ischemia,hypoxia and viral infection.The primary structure,including N-terminal sequence,C-terminal sequence,PMF,accurate molecular mass,and disulfide bonding pattern of recombinant human neuregulin,have been identified by ESI-Q-TOF MS,Autoflex MALDI-TOF MS,9.4T Apex Q-FT MS and Ultraflex Ⅲ MALDI-TOF/TOF combining with two e]ymatic digestion.A abnormal peptide impurity in this drug was found and sequenced by Q-TOF MS and TOF/TOF MS,this is useful for the product quanlity control.
Neuregulin plays an important role in heart structure and function.Research discovered that recombinant neuregulin could reduce the degree of damage on myocardial cells caused by ischemia,hypoxia and viral infection.The primary structure,including N-terminal sequence,C-terminal sequence,PMF,accurate molecular mass,and disulfide bonding pattern of recombinant human neuregulin,have been identified by ESI-Q-TOF MS,Autoflex MALDI-TOF MS,9.4T Apex Q-FT MS and Ultraflex Ⅲ MALDI-TOF/TOF combining with two e]ymatic digestion.A abnormal peptide impurity in this drug was found and sequenced by Q-TOF MS and TOF/TOF MS,this is useful for the product quanlity control.
出处
《分析测试学报》
CAS
CSCD
北大核心
2007年第z1期35-37,共3页
Journal of Instrumental Analysis
基金
北京市自然科学基金项目(2062022)
