摘要
Bovine urine was centrifuged.Added the internal standard(ZER-d4 and DES-d8) into the supernate.The extract was cleaned up by two immunoaffinity columns respectively.A C8 analytical column was used and the analytes were chromatographed by acetonitrile-water(70:30,v/v).With HPLC-MS/MS,data acquisition was achieved by using atmospheric pressure chemical ionization (APCI) in negative scan mode and multiple reaction monitoring (MRM) determination mode.Quantification was made by internal standarad method.The limits of determination of DES and DEN are 0.05 ng·g-1,the recovery level was 80 %-106% between 0.5 and 10 ng·g-1.The limits of determination of HEX and ZER are 0.025 ng·g-1,the recovery level was 83%-104% between 0.25 and 5 ng·g-1.
Bovine urine was centrifuged.Added the internal standard(ZER-d4 and DES-d8) into the supernate.The extract was cleaned up by two immunoaffinity columns respectively.A C8 analytical column was used and the analytes were chromatographed by acetonitrile-water(70:30,v/v).With HPLC-MS/MS,data acquisition was achieved by using atmospheric pressure chemical ionization (APCI) in negative scan mode and multiple reaction monitoring (MRM) determination mode.Quantification was made by internal standarad method.The limits of determination of DES and DEN are 0.05 ng·g-1,the recovery level was 80 %-106% between 0.5 and 10 ng·g-1.The limits of determination of HEX and ZER are 0.025 ng·g-1,the recovery level was 83%-104% between 0.25 and 5 ng·g-1.
出处
《分析测试学报》
CAS
CSCD
北大核心
2007年第z1期93-95,共3页
Journal of Instrumental Analysis
