麦叶蜂幼虫感染异小杆线虫的时间-剂量-死亡率模型研究

Study on Time-dose-mortality Model of Dolerus tritici Infected Heterorhabditis indica

在异小杆线虫LN2品系对麦叶蜂5龄幼虫的小规模敏感性试验基础上,进行了室内毒力测定实验,利用时间-剂量-死亡率分析技术对生测数据进行了处理。结果表明,48 h该品系的LD50为1 840线虫/虫。在9 000线虫/虫、4 500线虫/虫、2 250线虫/虫、1 130线虫/虫、560线虫/虫、280线虫/虫6个浓度梯度的处理下,LT50依次为41、44、47、51、56和61 h。和几率分析技术相比,时间-剂量-死亡率分析兼顾时间效应与剂量效应,可以用于昆虫病原线虫毒力评价。

On the basis of a small-scale sensitivity text,the indoor virulence bioassay of Heterorhabditis indica was carried out on Dolerus tritici,and the data were processed by time-dose-mortality(TDM) model.The results showed that the LD50 of nematode strain LN2 was 1840 infective juveniles per insect larvae at 48 hours,and the LT50 of this strain were 41 hr.,44 hr.,47 hr.,51 hr.,56 hr.and 61 hr.in the dosages order of 9,000 IJ per larvae,4,500 IJ per larvae,2,250 IJ per larvae,1,130 IJ per larvae,560 IJ per larvae and 280 IJ per larvae.In conclusion,TDM model technology considers both time and dosage effects compared with Probability Analysis Technology,and adapts to the virulence evaluation of Entomopathologenic nematode.