紫薇花粉萌发特性研究

Pollen Viability of Lagerstroemia indica

以5个花色(白色、紫色、玫红、浅红色和浅紫色)紫薇的花粉为材料,分别用I2-Kl染色法、TTC(2,3,5-氯代三苯基四氮唑)染色法和离体萌发法测定紫薇花粉活力.结果表明:蔗糖和硼酸影响紫薇花粉的萌发,20 mg·L-1的蔗糖和2.0 mg·L-1的硼酸对白色紫薇花粉萌发有促进作用;TTC染色法和I2-KI染色法不能准确表示紫薇花粉的生活力,染色5～8 h后的染色率仅为5.26%～18.57%;与对照相比,低温(1～3℃)贮藏2～5 d后,染色率变化不大.当温度为2～5℃、相对湿度为30%～50%时,紫薇花粉可以贮藏25 d以上.

Rates of germination of 5 Lagerstroemia indica with different colors(white,purple,roseate,light red,and lilac)were determined by TTC and I2-KI dyeing,and culturing in vitro methods.The results showed that the concentration of sucrose and boric acid were the important factors for the germination of pollen of L.indica,either sucrose(20 mg·L-1) or H3BO3(2.0 mg·L-1)could promote the germination;The vigor of the pollen was hard to be expressed trough either TTC or I2-KI dyeing,the rates of dyed pollen for 5～8 h reached only 5.26%～18.57%;The rates of dyed pollen didn't change much after stored in 1～3℃ for 3 days;The pollens can be stored in 2～5℃,30%～50% humidity for 25 d.