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GCS对急性炎性大鼠脑内c-fos表达的影响

Effects of Glucocorticoids on c-fos Express in the Brain of Rats Following Acute Inflammative Pain
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摘要 目的为探讨糖皮质激素(GCS)对脑内神经细胞的作用提供形态学资料。方法动物行为学观察、免疫组化法神经细胞染色和形态计量学方法。结果在大鼠后爪掌面皮下注射2.5%甲醛20μl可造成注射处急性持续性炎症。1h后,双侧中缝大核、丘脑、大脑皮层fos样免疫阳性神经元总数明显上升。用地塞米松预处理2h后可使相应部位FLI神经元数明显降低,具有剂量依赖性。外周水肿、疼痛表现与脑内FLI细胞数呈正相关。用糖皮质激素受体阻断剂RU486可部分反转GCS的效应。结论①伤害性刺激可引起中枢神经系统c-fos广泛表达;②GCS对中枢神经元c-fos表达的效应有差异性。 Objective To provide the morphologic information about glucocorticosteriod(GCS) effect on brain neurons.Methods Observation of animal ethology,nerve cells staining by immunohistochemistry and morphometry methods was performed.Results 2.5% formalin(20μl) injection into the plantar surface of rat hind paw could induce acute durative inflammation near area of the injection.After 1 hour the total number of fos-like immunoreactive(fos-LI) neurone in thalamencephalon and cerebral cortex increased obviously.But the number of FLI neuron was significantly reduced after 2 hours carulon pretreatment,and it was dose dependent.The number of FLI neuron had the positive correlation to the edema in peripheral tissue and the pain behavior.RU486,the blocking agent of glucocorticoid receptor,could reverse partly the effect of GCS.Conclusion ①c-fos is expressed widely in central nervous system after the nocuous stimulation;②GCS induced effect on central nervous system of c-fos expression is different
作者 刘洪国 于剑锋 张杰 李磊
机构地区 潍坊医学院解剖学教研室
出处 《潍坊医学院学报》 2007年第1期47-49,Ⅳ,共4页 Acta Academiae Medicinae Weifang
关键词 地塞米松 炎症 疼痛 C-FOS 脑 大鼠 Dexamethasone Inflammation Pain c-fos Brain Rats
分类号 R322.8 [医药卫生—人体解剖和组织胚胎学]
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参考文献11

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二级参考文献7

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潍坊医学院学报
2007年 第1期

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