盐酸普罗帕酮注射液对缺血再灌注损伤心肌细胞钙离子和氧自由基的影响

The Empirical Study of Effect of Propafenone on Ca^(2+) and Oxygen Free Radical of I/R Myocardial Cell

目的研究盐酸普罗帕酮对缺血再灌注损伤心肌细胞钙离子和氧自由基影响及其发生的可能机制。方法体外培养心肌细胞,建立缺血再灌注损伤模型,激光共聚焦显微镜观察细胞内钙离子荧光染色;722可见光分光广度计检测丙二醛(MDA)、超氧化物歧化酶(SOD)的含量;透射电镜观察心肌细胞超微结构的变化。结果盐酸普洛帕酮注射液可以使培养液中的荧光像素值和染色面积降低,可以使缺血再灌注心肌细胞培养液中的SOD升高,MDA降低,并且在药物浓度为40%的时候效果达到最佳。结论盐酸普洛帕酮注射液对缺血再灌注损伤的心肌细胞有保护作用,最佳药物浓度为40%.

Objective To study the of effect of propafenone on Ca2+ and oxygen free radical of I/R myocardial cell and the potential mechanism.Methods To cultivate myocardial cell in vitro,establish I/R model and measure the fluorescence stain of the intracellular calcium ion by laser scanning confocal microscope,measure MDA,SOD in the culture fluid by 722 visible range spectrophotometer;observe ultramicrostructure of myocardial cell by transmission electron microscope.Results Propafenone can decrease the value of fluorescence picture element and positive Ca2+ stain areas of ischemia-reperfusion injury of myocardial cell,can raise SOD and reduce MDA of I/R myocardial cell,and the effect reached the best when the concentration was 40%.Conclusion Propafenone has a protection on I/R isolated myocardial cell and it's best concentration is 40%.