IL-1β反义RNA真核表达载体的构建

The Construction of IL-1β Antisense RNA Eukaryon Expression Vectors

目的通过构建IL-1β反义RNA真核表达载体,为进一步研究IL-1β奠定了基础。方法采用RT-PCR法分别获取IL-1β的两段基因序列,利用基因工程方法构建IL-1β反义RNA真核表达载体,并对其进行反复PCR鉴定、单酶切鉴定和DNA序列分析。结果经最终DNA序列分析证实,成功构建了人IL-1β反义RNA真核表达载体。结论本研究为IL-1β尤其是肿瘤微环境中IL-1β作用的研究提供了证据。

Objective To further analyze the effect of the IL-1β through recombinant IL-1β antisense RNA eukaryon expression vectors.Methods Two segments of IL-1β gene was amplificated by the reverse transcription-polymerase chain reation(RT-PCR),and were used to to prepare antisense RNA expression vectors against human IL-1β?The gene inserted into the cloning vectors was identified through polymerase chain reation(PCR) assay,single restriction enzyme digestion,DNA sequencing.Results Through the final DNA sequencing,we have successfully constructed IL-1β antisense RNA expression vectors.Conclusion This study sets up a foundation for exploring the effects of IL-1β especially in tumor microenvironment.