基于光纤束传感器结构的激光诱导荧光检测系统的研制

Development of a Laser-Induced Fluorescence Detection System Based on Optical Fiber Beam Sensor

基于Y型光纤束传感器结构以及激光诱导荧光技术,以半导体激光器(中心波长473nm)为激发光源,自行研制了微流控芯片激光诱导荧光检测系统.该系统具有体积小、重量轻、成本低、灵敏度高、抗干扰能力强等特点.从激光诱导荧光光纤传感检测系统的结构出发,分析了荧光信号强度与荧光效率、入射光强度、物质的摩尔吸收系数以及溶液浓度的定量关系.并且以荧光素钠为试剂,作为检测物质对该系统性能进行了评价,在检测池尺寸为35mm×15mm时,最小检出限达到3×10-12mol/L,同时得到检测物质的荧光信号强度与溶液的浓度成线性关系.

Based on Y mode optic fiber beam sensor and the technology of laser-induced fluorescence,a miniaturized laser-induced fluorescence optical fiber detection system using solid-state laser(473 nm) as excitation source is developed.The advantages of the laser-induced fluorescence detection system are compact,small size,low cost,high sensitivity and high anti-disturbing.From the structural of laser-induced fluorescence optic fiber detection system,we analyze the intensity of fluorescence signal,which has a ration relationship with fluorescence efficiency,the intensity of incidence ray,the molar absorptive and solution concentration.Using Sodium Fluorescein as the test sample,when the dimension of detection tank is 35 mm×15 mm,the detection limit is 3×10-12mol/L.At the same time,we concluded that the intensity of fluorescence signal and solution concentration have a linearity relation.