心复康对心肌梗死大鼠心室重塑促血管生成的影响

Effect of XinFuKang on left ventricular remodeling and enhancing angiogenesis after myocardial infarction in rats

[目的]观察中药心复康对心肌梗死大鼠促血管新生的影响,探讨中药对心室重塑的保护作用。[方法]采用冠状动脉结扎法制作心肌梗死模型,术后随机分为假手术组、模型组、中药高剂量组和中剂量组,每组12只。中药高剂量[81g/(kg·d)]和中剂量组[40.5g/(kg·d)]给予中药心复康水煎剂灌胃,假手术组和模型组每天等量生理盐水灌胃。28d后处死动物,病理切片观察心脏微观结构改变;免疫组化方法计数心肌梗死灶、边缘区新生血管数。[结果]中药组大鼠心肌梗死面积明显缩小,梗死灶及周围组织中微血管密度、明显高于模型组及假手术对照组。[结论]在大鼠心肌梗死模型中,中药组能够有效促进微血管新生,减少胶原纤维的生成,减轻心肌梗死后心室重塑。

[Objective] To study the impact of XinFuKang on left ventricular remodeling (LVRM) and angiogenesis after myocardial infarction (MI) in rats. [Methods] The model of myocardial infarction was induced by ligating the descending left coronary artery in male rats. Twenty-four hours later, 48 survied rats with AMI were randomly divided into myocardial infarction control group, sham-operated group, Xinkukang middle dose group and Xinfukang high dose group. After four weeks of therapy,cardia tissue section were observed by immunohistochemistry. [Results] Compared to sham operated, Xinfukang high dose group can enhance angiogenesis and inhibit collagen fibers increased and disordered. [Conclusion] Xinfukang can prevent LVRM after MI in rats, prohibit collagen fibers hyperplasia and enhance angiogenesis.