供受体混合骨髓移植GVHD反应的动物实验研究

Study on GVHD of mixed Bone Marrow Transplantation in Experimental Animal Models

目的观察混合骨髓移植的GVHD反应。方法以急性放射病BALB/C小鼠(H-2d)为受鼠,C57BL/6(H-2b)小鼠为供鼠。供体鼠骨髓和脾细胞中混合一定比例的受体鼠脾脏细胞,进行混合骨髓移植。观察移植后小鼠死亡率、白细胞系造血重建、脾结节、体内混合淋巴细胞培养、嵌合体及病理学改变,观察混合骨髓移植方式的GVHD反应。结果异基因骨髓移植组发生明显的GVHD反应。供受鼠脾脏3∶1混合骨髓移植不能明显降低GVHD反应,供受鼠脾细胞比例1∶1和2∶1移植组可以明显减轻GVHD反应。表现为外周血白细胞及骨髓造血恢复快,体内混合淋巴细胞反应降低及嵌合体百分率升高。结论混合骨髓移植方式可以降低GVHD反应,并与同基因和异基因脾细胞比例有关。

Objective To observe GVHD reaction in mixed BMT.Methods BALB/C mice with acute radiation disease were used as recipient mice models,C57BL/6 mice were taken as donor mice.C57BL/6 mice bone marrow and spleen cells mixed with certain percent of syngeneic spleen cells were underwent mixed BMT to observe their death rate,hematopoietic recovery of WBC lineage,spleen node count,mixed lymphocyte reaction in vivo,chimerism and pathological changes.Results GVHD was relatively strong in non-mixed BMT groups,the group mixed by 3∶1 BMT after activation could not alleviate GVHD,whereas the mixed by 1∶1 BMT group or by 2∶1 group with high percentage of syngeneic spleen cells could lessen the extent of GVHD,as manifested by the quick recovery of peripheral blood WBC count and bone marrow GM-CFU formation,by the alleviation of mixed lymphocyte reaction in vivo as well as by the increase of chimeric rate.Conclusion Mixed BMT could lower the GVHD reaction,which was related to the ratio of syngeneic to allogeneic spleen cells.