摘要
目的构建含有人类视网膜母细胞瘤94基因(Rb94基因)的腺病毒表达载体,研究其联合γ-辐射抑制人恶性肿瘤细胞生长的作用。方法从人类胚胎中提取总RNA,经逆转录得到目的cDNA,用PCR技术扩增目的基因片段,将其连接入载体pENTRTM1A,然后经LR重组再转入到表达载体pAd/CMV/V5-DESTTM中,对重组体进行鉴定。然后联合γ-辐射作用于乳腺癌细胞,观察细胞生长曲线的变化。结果成功构建了含Rb94基因的腺病毒表达重组体并通过鉴定,联合γ-辐射作用于乳腺癌细胞后有更强的抑制效应。结论本实验构建的人Rb94基因重组腺病毒表达质粒是成功的,与γ-辐射联合共同作用于肿瘤细胞有更强的抑制肿瘤细胞生长的作用。
Objective To construct recombinant Rb94 gene adenovirus expression vector and to study its effect wirh γ-radiation in inhibiting the growth of breast cancer.Methods Total RNA was extracted from human embryo and reversed transcript to cDNA.Rb94 gene fragment was amplified by PCR and linked to pENTRTM1A vector.Rb94 gene was recombinated with pAd/CMV/V5-DESTTM by LR recombinant and identified.Combined with γ-radiation,if produces an inhibiting effect on breast cancer cells.Results The recombinant Rb94 gene adenovirus expression vector was constructed successfully.The combined effect is better in inhibiting the malignant cells.Conclusion The recombinant Rb94 gene adenovirus expression vector can be used to investigate the function of Rb94 gene.Combination effect is stronger in inhibiting the growth of human malignant tumor cells.
出处
《中国辐射卫生》
北大核心
2007年第3期277-279,共3页
Chinese Journal of Radiological Health
基金
天津市自然科学基金资助项目(编号:05YFTMJC11200)
