胎盘Hofbauer细胞的体外培养和鉴定

Culture in vitro and identification of Hofbauer cells from human placenta

目的 通过对早孕胎盘绒毛膜Hofbauer细胞的分离,纯化和培养,寻找一种稳定、简便的,可获得较高纯度Hofbauer细胞的培养方法,以满足后续实验要求.方法 通过胰酶/胶原酶联合消化法对妊娠6～10周绒毛组织进行消化,获得单细胞悬液,根据不同细胞贴壁时间的差异,对Hofbauer细胞进行纯化,并通过免疫组织化学法对Hofbauer细胞进行鉴定.结果 获得了较高纯度的胎盘Hofbauer细胞,免疫组化法鉴定CD68阳性,细胞生长状态良好.结论 利用胰酶/胶原酶联合消化法及差异贴壁法可以获得满意的人胎盘Hofbauer细胞.

Objective To find out a stable and convenient method to separate,purify and cultivate Hofbauer cells form human placenta in early stage of pregnancy.Methods In trypsin/collagenase combined digestion method,single cell suspension was obtained from healthy villous tissues of placenta in 6 ~ 10 weeks of gestation.According to diffidence in adherence time, Hofbauer cells in human placenta were separated and purified.DMEM culture medium containing 10% FBS was used for cell culture.The cells cultivated were identified by using immunohistochemical staining.Results Highly purified human placental Hofbauer cells were obtained,and the cells identified by using immunohistochemical staining,CD68 was positive and cell growth was better.Conclusion Using trypsin/collagenase combined digestion and diffidence in adherence time techniques,human Hofbauer cells from placenta can be obtained satisfactorily by culture in vitro.