湖南苗族人群载脂蛋白AⅤ基因多态性与血脂的关系

The Relationship Between the Polymorphism of Apolipoprotein AⅤ Gene and the Levels of Serum Lipids in Hunan Miao Population

目的研究中国湖南苗族人群载脂蛋白AⅤ基因S19W和SNP4多态性与血脂的关系。方法收集湖南湘西无血缘关系的苗族健康人241例,用聚合酶链反应—限制性片段长度多态性分析S19W和SNP4单核苷酸多态。分析基因型与血脂的关系。结果S19W位点都为56C/C型。SNP4位点具有-12238T/C和T/T和C/C三种基因型,等位基因T、C频率分别为0.45和0.55。苗族-12238T/C型的人群血浆甘油三酯显著高于其他基因型人群(T/C型、T/T型和C/C型分别为2.15±1.52、1.63±0.89和1.41±0.84mmol/L,P<0.05),低密度脂蛋白水平亦显著高于其他基因型人群(T/C型、T/T型和C/C型分别为2.93±0.84、2.44±0.70和2.37±0.85mmol/L,P<0.05)。结论载脂蛋白AⅤ基因S19W位点在湖南苗族健康人群中为非多态性位点。载脂蛋白AⅤ基因SNP4位点在湖南苗族健康人群中为多态性位点,T/C型血浆甘油三酯和低密度脂蛋白水平高于其他基因型。

Aim To investigate the single nucleotide polymorphism (SNP) SNP4 and S19W of the apolipoproteiin AⅤ (ApoAⅤ) gene possible association with serum lipids in Hunan Miao population. Methods 241 normal subjects were collected to detemine the genotypes by using polymerase reaction restriction fragments length polymorphisms. Results Only one genotype, 56C/C of S19W was found in 241 subjects. Three genotypes, -12238T/C, T/T and C/C of SNP4 were found in 241 subjects. Allelic frequencies of T and C were 0.45 and 0.55. The level of serum triglyceride (TG) in subjects with T/C genotype were significantly higher than those in subjects with other genotypes (T/C vs T/T vs C/C: 2.15±1.52 mmol/L vs 1.63±0.89 mmol/L vs 1.41±0.84 mmol/L, P<0.05). The level of serum low density lipoprotein (LDL) in subjects with T/C genotype was significantly higher than those in subjects with other genotypes too (T/C vs T/T vs C/C: 2.93±0.84 mmol/L vs 2.44±0.70 mmol/L vs 2.37±0.85 mmol/L, P<0.05). Conclusions S19W is not a polymorphism place in ApoAⅤ gene of Hunan Miao population. The polymorphism of SNP4 in ApoAⅤ gene is associated with level of serum lipids in Hunan Miao population. T/C type might be associated with higer level of serum TG and LDL (P<0.05).